Purification and properties of haloalkane dehalogenase from Corynebacterium sp. strain m15-3
نویسندگان
چکیده
منابع مشابه
Crystal structure and site - directed mutagenesis analyses of haloalkane 1 dehalogenase LinB from Sphingobium sp .
To whom correspondence should be addressed: Masaru Tanokura, Department of Applied 11 Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of 12 Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan Tel: 81-3-5841-5165; Fax: 13 81-3-5841-8023; E-mail: [email protected] 14 Copyright © 2013, American Society for Microbiology. All Rights Reserved. J. Bact...
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Enzymes II(a) and II(b), which catalyze the conversion of epichlorohydrin (ECH) to 3-chloro-1,2-propanediol (MCP), were purified from Corynebacterium sp. strain N-1074, which catalyzes the formation of (R)-MCP from prochiral 1,3-dichloro-2-propanol via ECH. The specific activity of enzyme II(a) for the formation of MCP from ECH was about 6.4-fold higher than that of enzyme II(b). Both enzymes c...
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Haloalkane dehalogenase converts halogenated alkanes to their corresponding alcohols. The active site is buried inside the protein and lined with hydrophobic residues. The reaction proceeds via a covalent substrate-enzyme complex. This paper describes a steady-state and pre-steady-state kinetic analysis of the conversion of a number of substrates of the dehalogenase. The kinetic mechanism for t...
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Haloalkane dehalogenases (HLDs) have recently been discovered in a number of bacteria, including symbionts and pathogens of both plants and humans. However, the biological roles of HLDs in these organisms are unclear. The development of efficient HLD inhibitors serving as molecular probes to explore their function would represent an important step toward a better understanding of these interest...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1987
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.169.9.4049-4054.1987